Atcc hl-60

Article Snippet: HL-60 culture under DMSO and ATRA exposure HL-60 cells (CCL-240, ATCC, Manassas, VA) were either cultured in RPMI 1640 containing with 10% heat-inactivated fetal bovine serum (FBS), 1% penicillin/streptomycin antibiotics or RPMI 1640 containing 2% FBS, 2% Nutridoma and 5 mL % penicillin/streptomycin antibiotics at 37 °C in 5%

All amounts and volumes are given on a per well basis. 1. The day of transfection, count the cells to determine culture density. Plate 1 x 105 cells per well in 0.5 ml of There is no reported evidence by the originators of HL-60 (33), the American Type Culture Collection (ATCC) (Rock-ville, Md.), or the European Collection of Cell Cultures See full list on sigmaaldrich.com The NCI-60 cancer cell line panel is a group of 60 human cancer cell lines used by the National Cancer Institute (NCI) for the screening of compounds to detect potential anticancer activity. HL-60: Part: WHOLE: Record UNII: RC2DVQ0F6O.

10.03.2021

Subsequent exposure of the … DNA taken from HL-60 (ATCC® CCL-240 ™) Cell Line. Cell Line Description: The HL-60 cell line is a human acute myeloid leukemia cell line. The HL-60 cell line is included in the genetic alteration cell panel MYC (ATCC® TCP-1035 ™). Spin down before use. Verified mutation in parental cell line: MYC amplification. Biosafety Level 1 Biosafety classification is based on U.S. Public Health Service … hl-60-luc2 atcc ® ccl-240-luc2 ™ frozen 1.0 mL For-Profit: $1,310.00 Non-Profit: $825.30 hl-60/s4 atcc ® crl-3306 ™ frozen 1.0 mL For-Profit: $632.00 Non-Profit: $537.20 HL-60 cells (white arrow) and MDA-MB-231 cells (red arrow) passing through the magnetophoretic separation module under various focusing buffer flow rates and magnetic field strength conditions of (c) 3 mL/h and 0 mT, (d) 3 mL/h and 20 mT, and (e) 5 mL/h and 20 mT. (f – i) Enlarged images of cells during separation from Figure 2 e.

While HL-60 and HL-60/S4 are recognized as different cell lines by ATCC, they are both used for similar studies [22,24]. It is notable that despite the HL-60/S4

The HL-60 cell line is a human leukemia cell line that has been used for laboratory research on blood cell formation and physiology. HL-60 proliferates continuously in suspension culture in nutrient and antibiotic chemicals. The doubling time is about 36–48 hours. ATCC Genome Portal.

HL-60/MX1 (see ATCC CRL-2258) cells were selected and subcloned in 1987 for resistance to 39 nM mitoxantrone, an anthracenedione antitumor agent. Subsequent exposure of the HL-60/MX1 cells to higher concentrations of mitoxantrone led to the emergence of cells capable of growth at a concentration of 190 nM.

Both cell lines express monocyte-specific esterase (MSE) and MLL-AF9 fusion m … (33), the American Type Culture Collection (ATCC) (Rock-ville, Md.), or the European Collection of Cell Cultures (ECACC) (Porton Down, United Kingdom) that HL-60 cells are infected by pathogenic The protocols I have read seem straightforward on differentiating HL-60 cells, however I am not having luck using 1.3% DMSO and/or 1uM ATRA (all trans retinoic acid). I culture in 1640 RPMI The human promyelocytic leukemia cell line HL-60 (ATCC® CCL240™) was purchased from the American Type Culture Collection (ATCC, USA). HL-60 cells were commonly cultured in RPMI 1640 medium supple-mented with 10% (v/v) fetal bovine serum containing 100 U/ml penicillin and 100 U/ml streptomycin at 37 °C in a humidified atmosphere with 5% CO 2.

ZERO BIAS - scores, article reviews, protocol conditions and more Bioz Stars score: 99/100, based on 2 PubMed citations. Cell culture Human acute promyelocyte leukemia HL-60 cells and acute myeloid leukemia KG1a cells were purchased from the American Type Culture Collection (ATCC)(Manassas, VA, USA) and cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) (Gibco, USA), penicillin (100 U/mL, Gibco), and streptomycin (100 µg/mL, Gibco). .. Article Title: A Glycovariant of Human CD44 is … 25/05/2017 ATCC is the complete solution supplier for toxicology From basic research through discovery and development to product testing −Continuous cell lines −Primary cells −hTERT-immortalized primary cells −upcyte ® hepatocytes (ATCC ® ACS-9000 ™) Portfolio features −Reliability −Fully characterized cells −Optimized growth protocols −Scalabilty into all aspects of the toxicology workflow −Biological … The prostate cancer cell lines LNCaP (ATCC, Manassas, VA, USA; ATCC CRL-1740) and PC3 (ATCC CRL-1435) as well as the myeloid cell lines THP-1 (ATCC TIB-202) and HL-60 (ATCC CCL-240) wereusedinthisstudy. HL-60 is a promyelocytic cell line derived by S.J. Collins, et al.

Although the cells are capable of reverting to the parental phenotype, most passages maintain a high degree of eosinophil differentiation.Eos-HL-60 and HL-60 have been shown to be identical genetically by STR . July 2018 Page . 2. of .

All experi-ments were performed only … HL-60 cells were treated with different concentration of MK886 (10, 25, 50 µmol/L) for 24 h. Flow cytometry, Western blot and ELISA were used to measure cell cycle, cyclin D1, mPGES-1, PGE(2), 20/03/2018 Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH Human HL-60 cells (ATCC-No: CCL-240) were isolated from a patient with AML (acute myeloid leukemia) and express FLT3 wild type. A Hematoxylen-Eosin stained paraffin section of a subcutaneous HL-60 xenograft is shown on the right. As routine quality controls, the cells are regularly checked for Mycoplasma contamination and authenticity (via STR DNA Typing). Expression data using western … Article Snippet: Cell lines The mycoplasma-free AML U937 (ATCC CRL-1593.2; French-American-British/FAB phenotype M5, monoblast), NB4 (M3, promyelocyte) [ ], HL-60 (ATCC 240-CCL; M2, myeloblast) and THP-1 (ATCC TIB-202; M5, monoblast) cells were cultured in complete RPMI 1640 medium supplemented with 5% FCS in a 5% CO2 humidified atmosphere at Human Aml Cell Lines Hl60, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations.

Exosomes. ATCC offers exosomes derived from well … Article Snippet: The Ltx-sensitive human promyelocytic leukemia cell line HL-60 (ATCC CCL 240; American Type Culture Collection, Bethesda, Md.), was used for all experiments. Techniques: Incubation, Plasmid Preparation. Journal: Infection and Immunity. Article Title: Actinobacillus actinomycetemcomitans Leukotoxin Induces Apoptosis in HL-60 Cells. doi: Figure Lengend Snippet: … HL-60 from ATCC.

DMEM with 2mM L-glutamine (cellgro Cat# 10-013-CM) 2. Fetal Bovine Serum (cellgro Cat# 35-016-CV) 3. T75 & T225 culture flasks 4. Graduated pipets (1, 5, 25mL) 5. Penicillin-Streptomycin Solution (100X) (Cellgro Cat# 30-002 … ATCC HL-60, Promyelocytic Leukemia, Frozen . Manufacturer: ATCC CCL240 HL-60 cells spontaneously differentiate and differentiation can be stimulated by butyrate, hypoxanthine, phorbol myristic acid (PMA, TPA), dimethylsulfoxide (DMSO, 1% to 1.5%), actinomycin D, and retinoic acid. The cells exhibit phagocytic activity and responsiveness to chemotactic stimuli.

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DNA into HL-60 Human lymphoblasts (ATCC Cat. No. CCL-240) using Lipofectamine To transfect HL-60 cells in different tissue culture formats, vary the amounts of Lipofectamine ™ LTX Reagent, DNA, cells, medium and PLUS™ Reagent used in proportion to the relative surface area, as shown in the table (amounts given on a per well basis). Culture vessel Surface area per well1 Volume plating medium …

Bone marrow. Monocyte.

HL-60-Luc2 (ATCC® CCL-240-LUC2™). This luciferase expressing cell line was derived from parental line CCL-240 by transduction with lentiviral vector

Characteristics. Derivation. HL-60/MX1 is a mitoxantrone resistant derivative of the HL-60 cell line (see ATCC CCL-240) which was obtained from peripheral blood Clone 15 HL-60 (ATCC® CRL-1964™). This clone, picked from soft agar, was selected for its ability to undergo eosinophilic differentiation when treated with DNA purified from ATCC CCL-240, HL-60, acute promyelocytic leukemia cell line . MoreLess. Pricing.

The HL-60 cell line is included in the genetic alteration cell panel MYC (ATCC® TCP-1035 ™). Spin down before use. Verified mutation in parental cell line: MYC amplification. Biosafety Level 1 Biosafety classification is based on U.S. Public Health Service … hl-60-luc2 atcc ® ccl-240-luc2 ™ frozen 1.0 mL For-Profit: $1,310.00 Non-Profit: $825.30 hl-60/s4 atcc ® crl-3306 ™ frozen 1.0 mL For-Profit: $632.00 Non-Profit: $537.20 HL-60 cells (white arrow) and MDA-MB-231 cells (red arrow) passing through the magnetophoretic separation module under various focusing buffer flow rates and magnetic field strength conditions of (c) 3 mL/h and 0 mT, (d) 3 mL/h and 20 mT, and (e) 5 mL/h and 20 mT.